Splenocyte proliferation and anaphylaxis induced by BSA challenge in a D-galactose-induced aging mouse model
نویسندگان
چکیده
We previously found a cross-reactive autoantibody that bound to bovine serum albumin generated in a D-galactose-induced aging mouse model. Also, we confirmed that other reducing sugars (glucose and fructose) could induce the formation of autoantibody, and only following subcutaneous injection, not oral or intraperitoneal administration. Mice that had never been exposed to bovine serum albumin produced an anti-bovine serum albumin autoantibody following repeated subcutaneous injection of D-galactose (D-gal). In this study, we investigated the involvement of the adaptive immune system in the production of this autoantibody. In particular, we examined bovine serum albumin-induced splenocyte proliferation and bovine serum albumin-induced active cutaneous and systemic anaphylaxis in D-gal-treated mice. We find our results particularly interesting: bovine serum albumin stimulates splenocyte proliferation and induces both active cutaneous and systemic anaphylaxis in D-gal-treated mice. In summary, our results suggest that adaptive immune response participates in the autoantibody formation against bovine serum albumin in D-gal-treated mice.
منابع مشابه
Effect of hydro alcoholic extract of Purslane (Portulaca Oleracea L.) on kidney of aging model induced by D-galactose in female mice
This study was conducted to evaluate the effect of Purslane hydro alcoholic extract on blood urea nitrogen (BUN), creatinine (Cr) and kidney histopathology of kidney aging model induced by D-galactose in female mice. In this experimental study, 72 female Naval Medical Research Institute (NMRI) mice (30-35 g) were randomly divided into six groups including: control, Purslane, D-galactose, D-gala...
متن کاملEffects of hydro-alcoholic extract of Vitex agnus-castus fruit on kidney of D-galactose-induced aging model in female mice
The aim of the present study was to evaluate the effect of a hydro-alcoholic extract of Vitex agnus-castus (VAC) fruit on blood urea nitrogen (BUN), creatinine (Cr) and, kidney histology of a female mouse model of D-galactose induced aging. In this experimental study, 72 NMRI mice were divided into 6 groups: control, VAC, D-galactose, D-galactose+VAC, aging, and aging+VAC. D-galactose was injec...
متن کاملProtective effect of metformin on D-galactose-induced aging model in mice
Objective(s): Metformin (Met), an antidiabetic biguanide, reduces hyperglycemia via improving glucose utilization and reducing the gluconeogenesis. Met has been shown to exert neuroprotective, antioxidant and anti-inflammatory properties. The present study investigated the possible effect of Met on the D-galactose (D-gal)-induced aging in mice. Materials and Methods: Met (1 and 10 mg/kg/p.o.), ...
متن کاملProtective effect of crocin against d-galactose-induced aging in mice
Objective: Aging is a multifactorial phenomenon, which attribute to different diseases and abnormalities in living systems. Oxidative stress, which is an important factor in aging, exacerbates this process via different mechanisms. Crocin (CR), one of the active components of saffron showed strong antioxidant effects. In the present study, anti-aging property of crocin was investigated in mice....
متن کاملP 36: Protective Effects of Crocin on D-Galactose Induced Aging Model in Human Neuroblastoma Cells
Introduction: D-galactose (D-gal) is well known as an appropriate agent to induced aging effects in the in vivo and in vitro models. In the present study, we selected crocin, the main constituent of Crocus sativus L. (Saffron), against D-gal cytotoxicity in human neuroblastoma SH-SY5Y cells. Materials and Methods: Cells were pretreated with crocin (25-500 µM) for 24 h and then expos...
متن کامل